A sturdy tripod is obviously the best but I very often use the 100 2.8 with a Monopod when in the field as it is easier/quicker to use. I also use this lens with a 1.4 tc (kenko pro) to give a bit more working distance for butterflies/dragonflies/insects.
As already stated, depth of field is the main problem with macro so you need to close down to f11-16 typically. I have no problems with shooting at ISO 400 if needed to get a reasonable shutter speed.
As 'Yelvertoft' has said, a lot depends on your subject - if you are shooting mega close-ups then a remote release and a sturdy tripod gives the best results.
Another tip is to set the lens at 0.48m - infinity if you do not need to get any closer as AF is a lot better than 0.31m - infinity ( but for mega close-ups use manual focus anyway).
For moving subjects takes loads of shots as you are bound to get Duffers, if you take 10 shots of a subject and nail just one that's all you need.
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