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Originally Posted by Roy C
If you convert a 16 bit image to a 8 bit and then back again to a 16 bit do you lose anything in the way of quality?
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Once you've converted to 8 bit, you can't get the "true" 16 bits back again. If you convert back, the 16 bit file will be an interpolation of the 8 bit data, not the original 16 bit data.
Whether you'd ever be able to tell the difference is another matter completely, I suspect not unless you are making large prints of images with exceptionally subtle colour variations.
You could convert to JPEG2000, lossless 16 bit format.
Duncan